​Publications

Mears Lab Papers

​Preprint​
  1. Hannigan MM, Fujioka H, Brett-Morris A, Mears JA, and Licatalosi DD. PTBP2-dependent alternative splicing regulates protein transport and mitochondria morphology in post-meiotic germ cells. bioRxiv 497461
Accepted
  1. Peng R*, Rochon K*, Hutson AN, Stagg SM#, Mears JA#. (2025) The Structure of the Drp1 Lattice on Membrane. J. Mol. Biol. 437:169125. PMID: 40185198
  2. Buckley YB, Stoll MSK, Hoppel CL, and Mears JA. (2025) Fis1 regulates mitochondrial morphology, bioenergetics, and removal of mtDNA damage in irradiated glioblastoma cells. J Cell Sci. 138: jcs.263459. PMID: 39704270
  3. Mears JA. (2024) Mitochondrial Biology: Unique Membrane Remodeling from the Matrix. Curr. Biol. 34: R564-586. PMID: 38889682
  4. Rochon K, Bauer BL, Roethler NA, Buckley Y, Su C-C, Huang W, Ramachandran R, Stoll MS, Yu EW, Taylor DJ, and Mears JA. (2024) Structural Basis for Regulated Assembly of the Mitochondrial Fission GTPase Drp1. Nat. Commun. 15:1328. PMID: 38351080
  5. Pérez-Jover I*, Rochon K*, Hu D*, Mahajan M, Mohan PM, Santos-Perez I, Gisasola JO, Martinez Galvez JM, Agirre J, Qi X, Mears JA, Shnyrova AV, and Ramachandran R. (2024) Allosteric control of dynamin-related protein 1 through a disordered C-terminal Short Linear Motif. Nat. Commun. 15: 52.  PMID: 38168038
  6. Zhang Z, Tringides ML, Morgan CE, Miyagi M, Mears JA, Hoppel CL, and Yu EW. (2023) High-resolution structural proteomics of mitochondria using the 'Build and Retrieve' methodology. Mol Cell Proteomics. 22: 100666. PMID: 37839702
  7. Watson DC, Bayik D, Storevik S, Moreino SS, Sprowls SA, Han J, Augustsson MT, Lauko A, Sravya P, Røsland GV, Troike K, Tronstad KJ, Wang S, Sarnow K, Kay K, Lunavat TR, Silver DJ, Dayal S, Joseph JV, Mulkearns-Hubert E, Ystaas LAR, Deshpande G, Guyon J, Zhou Y, Magaut CR, Seder J, Neises L, Williford SE, Meiser J, Scott AJ, Sajjakulnukit P, Mears JA, Bjerkvig R, Chakraborty A, Daubon T, Cheng F, Lyssiotis CA, Wahl DR, Hjelmeland AB, Hossain JA, Miletic H, Lathia JD. (2023) GAP43-dependent mitochondria transfer from astrocytes enhances glioblastoma tumorigenicity. Nat. Cancer. 4: 638-664. PMID: 37169842
  8. Bauer BL, Rochon K, Liu J, Ramachandran R, Mears JA. (2023) Disease-associated mutations in Drp1 have fundamentally different effects on the mitochondrial fission machinery. Hum. Mol. Genet. 32: 1975-1987. PMID: 36795043
  9. Robertson GL, Riffle SN, Patel M, Marshall A, Beasley H, Garza-Lopez E, Shao J, Vue Z, Stoll M, de Wet S, Theart R, Loos B, Hinton A Jr., Mears JA, and Gama V. (2023) DRP1-mediated mitochondrial fission is essential to maintain cristae morphology and bioenergetics. J. Cell Sci. 136: jcs260370 PMID: 36763487
  10. Attaway AH, Bellar A, Mishra S, Karthikeyan M, Sekar J, Welch N, Musich R, Singh SS, Kumar A, Menon A, King J, Langen R, Webster J, Scheraga R, Rochon K, Mears J, Prasad SVN, Hatzaglou M, Chakraborty AA, Dasarathy S. (2023) Adaptive exhaustion during prolonged intermittent hypoxia causes dysregulated skeletal muscle protein homeostasis. J. Physiol. 601: 567-606. PMID: 36533558
  11. Mears JA and Ramachandran R. (2022) Drp1 and the cytoskeleton: mechanistic nexus in mitochondrial division. Curr Opin Physiol. 29: 100574. PMID: 36406887
  12. Ducich NH, Cioffi G, Jin B, Barnholtz-Sloan JS, Mears JA, and Bedoyan JK. (2022) Solvent accessibility of E1α and E1β residues with known missense variations causing pyruvate dehydrogenase complex (PDC) deficiency: Impact on PDC-E1 structure and function. J. Inherit. Metab. Dis. 45: 557-570. PMID: 35038180
  13. Akinbiyi EO, Abramowitz LK, Bauer BL, Stoll M, Hoppel CL, Hsiao CP, Hanover JA, and Mears JA. (2021) Blocked O-GlcNAc cycling alters mitochondrial morphology, function, and mass. Sci. Rep. 11: 22106. PMID: 34764359
  14. Sha Z, Montano MM, Rochon K, Mears JA, Deredge D, Wintrode P, Szweda L, Mikita N and Lee I. (2021) A Structure and Function Relationship Study to Identify the Impact of the R721G Mutation in The Human Mitochondrial Lon Protease. Arch Biochem Biophys. 710:108983. PMID: 34228963
  15. Montecinos-Franjola F, Bauer BL, Mears JA, and Ramachandran R. (2020) GFP fluorescence tagging alters dynamin-related protein 1 oligomerization dynamics and favors protein crowding-mediated mitochondrial fission. Sci. Rep. 10: 14777. PMID: 32901052
  16. Clinton RW, Bauer BL, and Mears JA. (2020) Purification of dynamin-related protein 1 for structural and functional studies. Methods in Mol. Biol. 2159:41-53. PMID: 32529362
  17. Hoppins S, Lackner LL, Lee JE, and Mears JA. (2020) In vitro and in vivo assays for mitochondrial fission and fusion. Methods Cell Biol. 155: 491-518. PMID: 32183974
  18. Tiosano D, Mears JA, and Buchner DA. (2019) Mitochondrial Dysfunction in Primary Ovarian Insufficiency. Endocrinology. 160:2353-2366. PMID: 31393557
  19. Lu B, Kennedy B, Clinton RW, Wang E, McHugh D, Stepanyants N, Macdonald PJ, Mears JA, Qi X, and Ramachandran R. (2018) Steric interference from intrinsically disordered polypeptide regions controls dynamin-related protein 1 self-assembly during mitochondrial fission. Sci. Rep., 8: 10879. PMID: 30022112
  20. Tandler B, Hoppel CL, and Mears JA. (2018) Morphological pathways of mitochondrial division. Antioxidants., 7: E30. PMID: 29462856
  21. Francy CA, Clinton RW, Fröhlich C, Murphy C, and Mears JA. (2017) Cryo-EM Studies of Drp1 Reveal Cardiolipin Interactions that Activate the Helical Oligomer. Sci. Rep., 7: 10744. PMID: 28878368
  22. Clinton RW and Mears JA. (2017) Using scaffold liposomes to reconstitute lipid-proximal protein-protein interactions in vitro. J. Vis. Exp., 119: e54971. PMID: 28117823
  23. Macdonald PJ, Francy CA, Stepanyants N, Lehman L, Baglio A, Mears JA, Qi X, Ramachandran R. (2016) Distinct Splice Variants of Dynamin-related Protein 1 Differentially Utilize Mitochondrial Fission Factor as an Effector of Cooperative GTPase Activity. J. Biol. Chem., 291: 493-507. PMID: 26578513
  24. Clinton RW, Francy CA, Ramachandran R, Qi X, Mears JA. (2016) Dynamin-related Protein 1 Oligomerization in Solution Impairs Functional Interactions with Membrane-anchored Mitochondrial Fission Factor. J. Biol. Chem., 291: 478-92. PMID: 26578514
  25. Stepanyants N, MacDonald PJ, Francy CA, Mears JA, Qi X, and Ramachandran R. (2015) Cardiolipin's propensity for phase transition and its reorganization by dynamin-related protein 1 form a basis for mitochondrial membrane fission. Mol. Biol. Cell., 26: 3104-16. PMID: 26157169
  26. Francy CA*, Alvarez FJD*, Zhou L, Mears JA. (2015) The Mechanoenzymatic Properties of Drp1 in Nucleotide Induced Constriction of Lipid Bilayers. J. Biol. Chem., 290: 11692-703. PMID: 25770210
  27. MacDonald PJ, Stepanyants N, Mehrotra N, Mears JA, Qi X, Sesaki H, Ramachandran R. (2014) A dimeric equilibrium intermediate nucleates Drp1 reassembly on mitochondrial membranes for fission. Mol. Biol. Cell, 25:1905-15. PMID: 24790094
  28. Mears JA (2014) Mitochondrial biogenesis and quality control. In The Structural Basis of Biological Energy Generation. Ed. Hohmann-Marriott, M. Springer, Dordrecht, The Netherlands. pp. 451-76. Link
  29. Fröhlich C, Grabiger S, Schwefel D, Faelber K, Mears J, Rocks O, and Daumke O (2013) The crystal structure of human dynamin 1-like protein reveals an alternative oligomerization mode. EMBO J., 32: 1280-92. PMID: 23584531
  30. Jomaa A, Stewart G, Mears JA, Kireeva I, Brown ED, Ortega J (2011) Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor. RNA, 17: 2026–38. PMID: 21960487

​Papers from Jason's Post-Doctoral Studies

  1. Chappie JS, Mears JA, Fang S, Leonard M, Schmid SL, Milligan RA, Hinshaw JE, Dyda F (2011) A pseudo-atomic model of the dynamin polymer identifies a hydrolysis-dependent power stroke. Cell, 147: 209-22. PMID: 21962517
  2. Mears JA, Lackner L, Fang S, Ingerman E, Nunnari J, Hinshaw JE (2011) Conformational changes in Dnm1 support a contractile mechanism for mitochondrial fission. Nat. Struct. Mol. Biol., 18: 20-26. PMID: 21170049
  3. Schulz TA, Choi M-G, Raychaudhuri S, Mears JA, Ghirlando R, Hinshaw JE, Prinz WA (2009) Lipid-regulated sterol transfer between closely apposed membranes by oxysterol-binding protein homologues. J. Cell Biol., 187: 889-903. PMID: 20008566
  4. Mears JA, Hinshaw JE (2008) Visualization of dynamins. Methods in Cell Biol., 88, 237-256. PMID: 18617037
  5. Mears JA, Ray P, Hinshaw JE (2007) A corkscrew model for dynamin constriction. Structure, 15: 1190-1202. PMID: 17937909
  6. Ingerman E, Perkins EM, Marino M, Mears JA, McCaffery JM, Hinshaw JE, Nunnari J (2005) Dnm1 forms spirals that are structurally tailored to fit mitochondria. J. Cell Biol., 170: 1021-1027. PMID: 16186251

​Papers from Jason's Graduate Studies

  1. Mears JA*, Sharma MR*, Gutell RR, McCook AS, Richardson PE, Caulfield TA, Agrawal RK and Harvey SC (2006) A structural model for the large subunit of the mammalian mitochondrial ribosome. J. Mol. Biol., 358: 193-212. PMID: 16510155
  2. Mears JA, Stagg SM, Harvey SC (2005) Modeling large RNA assemblies using a reduced representation. In Handbook of RNA Biochemistry. Eds. Hartmann, RK, Bindereif, A, Schon, A and Westof, E. Wiley-VCH, Weinheim, Germany, pp. 546-559. Link
  3. Ivanov VI, Mears JA (2004) Using crosslinks to study ribosomal dynamics. J. Biomol. Struct. Dyn., 21: 691-698. PMID: 14769062
  4. Stagg SM*, Mears JA*, Harvey SC (2003) A Structural Model for the Assembly of the 30S Subunit of the Ribosome. J. Mol. Biol., 328: 49-61. PMID: 12683996
  5. Mears JA, Cannone JJ, Stagg SM, Gutell RG, Agrawal RK, Harvey SC (2002) Modeling a minimal ribosome based on comparative sequence analysis. J. Mol. Biol., 321: 215-234. PMID: 12144780

J Cell Biol Cover:
​Ring Around a Mitochondrion

NSMB Cover:
​Constricting Mitochondria

J Cell Sci Cover: Cell Biology of Mitochondria
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