Mears Lab Papers
Preprint
- Peng R*, Rochon K*, Stagg SM#, Mears JA#. (2024) The Structure of the Drp1 Lattice on Membrane. Submitted. bioRxiv 2024.04.04.588123
- Hannigan MM, Fujioka H, Brett-Morris A, Mears JA, and Licatalosi DD. PTBP2-dependent alternative splicing regulates protein transport and mitochondria morphology in post-meiotic germ cells. bioRxiv 497461
- Rochon K, Bauer BL, Roethler NA, Buckley Y, Su C-C, Huang W, Ramachandran R, Stoll MS, Yu EW, Taylor DJ, and Mears JA. (2024) Structural Basis for Regulated Assembly of the Mitochondrial Fission GTPase Drp1. Nat. Commun. 15:1328. PMID: 38351080
- Pérez-Jover I*, Rochon K*, Hu D*, Mahajan M, Mohan PM, Santos-Perez I, Gisasola JO, Martinez Galvez JM, Agirre J, Qi X, Mears JA, Shnyrova AV, and Ramachandran R. (2024) Allosteric control of dynamin-related protein 1 through a disordered C-terminal Short Linear Motif. Nat. Commun. 15: 52. PMID: 38168038
- Zhang Z, Tringides ML, Morgan CE, Miyagi M, Mears JA, Hoppel CL, and Yu EW. (2023) High-resolution structural proteomics of mitochondria using the 'Build and Retrieve' methodology. Mol Cell Proteomics. 22: 100666. PMID: 37839702
- Watson DC, Bayik D, Storevik S, Moreino SS, Sprowls SA, Han J, Augustsson MT, Lauko A, Sravya P, Røsland GV, Troike K, Tronstad KJ, Wang S, Sarnow K, Kay K, Lunavat TR, Silver DJ, Dayal S, Joseph JV, Mulkearns-Hubert E, Ystaas LAR, Deshpande G, Guyon J, Zhou Y, Magaut CR, Seder J, Neises L, Williford SE, Meiser J, Scott AJ, Sajjakulnukit P, Mears JA, Bjerkvig R, Chakraborty A, Daubon T, Cheng F, Lyssiotis CA, Wahl DR, Hjelmeland AB, Hossain JA, Miletic H, Lathia JD. (2023) GAP43-dependent mitochondria transfer from astrocytes enhances glioblastoma tumorigenicity. Nat. Cancer. 4: 638-664. PMID: 37169842
- Bauer BL, Rochon K, Liu J, Ramachandran R, Mears JA. (2023) Disease-associated mutations in Drp1 have fundamentally different effects on the mitochondrial fission machinery. Hum. Mol. Genet. 32: 1975-1987. PMID: 36795043
- Robertson GL, Riffle SN, Patel M, Marshall A, Beasley H, Garza-Lopez E, Shao J, Vue Z, Stoll M, de Wet S, Theart R, Loos B, Hinton A Jr., Mears JA, and Gama V. (2023) DRP1-mediated mitochondrial fission is essential to maintain cristae morphology and bioenergetics. J. Cell Sci. 136: jcs260370 PMID: 36763487
- Attaway AH, Bellar A, Mishra S, Karthikeyan M, Sekar J, Welch N, Musich R, Singh SS, Kumar A, Menon A, King J, Langen R, Webster J, Scheraga R, Rochon K, Mears J, Prasad SVN, Hatzaglou M, Chakraborty AA, Dasarathy S. (2023) Adaptive exhaustion during prolonged intermittent hypoxia causes dysregulated skeletal muscle protein homeostasis. J. Physiol. 601: 567-606. PMID: 36533558
- Mears JA and Ramachandran R. (2022) Drp1 and the cytoskeleton: mechanistic nexus in mitochondrial division. Curr Opin Physiol. 29: 100574. PMID: 36406887
- Ducich NH, Cioffi G, Jin B, Barnholtz-Sloan JS, Mears JA, and Bedoyan JK. (2022) Solvent accessibility of E1α and E1β residues with known missense variations causing pyruvate dehydrogenase complex (PDC) deficiency: Impact on PDC-E1 structure and function. J. Inherit. Metab. Dis. 45: 557-570. PMID: 35038180
- Akinbiyi EO, Abramowitz LK, Bauer BL, Stoll M, Hoppel CL, Hsiao CP, Hanover JA, and Mears JA. (2021) Blocked O-GlcNAc cycling alters mitochondrial morphology, function, and mass. Sci. Rep. 11: 22106. PMID: 34764359
- Sha Z, Montano MM, Rochon K, Mears JA, Deredge D, Wintrode P, Szweda L, Mikita N and Lee I. (2021) A Structure and Function Relationship Study to Identify the Impact of the R721G Mutation in The Human Mitochondrial Lon Protease. Arch Biochem Biophys. 710:108983. PMID: 34228963
- Montecinos-Franjola F, Bauer BL, Mears JA, and Ramachandran R. (2020) GFP fluorescence tagging alters dynamin-related protein 1 oligomerization dynamics and favors protein crowding-mediated mitochondrial fission. Sci. Rep. 10: 14777. PMID: 32901052
- Clinton RW, Bauer BL, and Mears JA. (2020) Purification of dynamin-related protein 1 for structural and functional studies. Methods in Mol. Biol. 2159:41-53. PMID: 32529362
- Hoppins S, Lackner LL, Lee JE, and Mears JA. (2020) In vitro and in vivo assays for mitochondrial fission and fusion. Methods Cell Biol. 155: 491-518. PMID: 32183974
- Tiosano D, Mears JA, and Buchner DA. (2019) Mitochondrial Dysfunction in Primary Ovarian Insufficiency. Endocrinology. 160:2353-2366. PMID: 31393557
- Lu B, Kennedy B, Clinton RW, Wang E, McHugh D, Stepanyants N, Macdonald PJ, Mears JA, Qi X, and Ramachandran R. (2018) Steric interference from intrinsically disordered polypeptide regions controls dynamin-related protein 1 self-assembly during mitochondrial fission. Sci. Rep., 8: 10879. PMID: 30022112
- Tandler B, Hoppel CL, and Mears JA. (2018) Morphological pathways of mitochondrial division. Antioxidants., 7: E30. PMID: 29462856
- Francy CA, Clinton RW, Fröhlich C, Murphy C, and Mears JA. (2017) Cryo-EM Studies of Drp1 Reveal Cardiolipin Interactions that Activate the Helical Oligomer. Sci. Rep., 7: 10744. PMID: 28878368
- Clinton RW and Mears JA. (2017) Using scaffold liposomes to reconstitute lipid-proximal protein-protein interactions in vitro. J. Vis. Exp., 119: e54971. PMID: 28117823
- Macdonald PJ, Francy CA, Stepanyants N, Lehman L, Baglio A, Mears JA, Qi X, Ramachandran R. (2016) Distinct Splice Variants of Dynamin-related Protein 1 Differentially Utilize Mitochondrial Fission Factor as an Effector of Cooperative GTPase Activity. J. Biol. Chem., 291: 493-507. PMID: 26578513
- Clinton RW, Francy CA, Ramachandran R, Qi X, Mears JA. (2016) Dynamin-related Protein 1 Oligomerization in Solution Impairs Functional Interactions with Membrane-anchored Mitochondrial Fission Factor. J. Biol. Chem., 291: 478-92. PMID: 26578514
- Stepanyants N, MacDonald PJ, Francy CA, Mears JA, Qi X, and Ramachandran R. (2015) Cardiolipin's propensity for phase transition and its reorganization by dynamin-related protein 1 form a basis for mitochondrial membrane fission. Mol. Biol. Cell., 26: 3104-16. PMID: 26157169
- Francy CA*, Alvarez FJD*, Zhou L, Mears JA. (2015) The Mechanoenzymatic Properties of Drp1 in Nucleotide Induced Constriction of Lipid Bilayers. J. Biol. Chem., 290: 11692-703. PMID: 25770210
- MacDonald PJ, Stepanyants N, Mehrotra N, Mears JA, Qi X, Sesaki H, Ramachandran R. (2014) A dimeric equilibrium intermediate nucleates Drp1 reassembly on mitochondrial membranes for fission. Mol. Biol. Cell, 25:1905-15. PMID: 24790094
- Mears JA (2014) Mitochondrial biogenesis and quality control. In The Structural Basis of Biological Energy Generation. Ed. Hohmann-Marriott, M. Springer, Dordrecht, The Netherlands. pp. 451-76. Link
- Fröhlich C, Grabiger S, Schwefel D, Faelber K, Mears J, Rocks O, and Daumke O (2013) The crystal structure of human dynamin 1-like protein reveals an alternative oligomerization mode. EMBO J., 32: 1280-92. PMID: 23584531
- Jomaa A, Stewart G, Mears JA, Kireeva I, Brown ED, Ortega J (2011) Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor. RNA, 17: 2026–38. PMID: 21960487
Papers from Jason's Post-Doctoral Studies
- Chappie JS, Mears JA, Fang S, Leonard M, Schmid SL, Milligan RA, Hinshaw JE, Dyda F (2011) A pseudo-atomic model of the dynamin polymer identifies a hydrolysis-dependent power stroke. Cell, 147: 209-22. PMID: 21962517
- Mears JA, Lackner L, Fang S, Ingerman E, Nunnari J, Hinshaw JE (2011) Conformational changes in Dnm1 support a contractile mechanism for mitochondrial fission. Nat. Struct. Mol. Biol., 18: 20-26. PMID: 21170049
- Schulz TA, Choi M-G, Raychaudhuri S, Mears JA, Ghirlando R, Hinshaw JE, Prinz WA (2009) Lipid-regulated sterol transfer between closely apposed membranes by oxysterol-binding protein homologues. J. Cell Biol., 187: 889-903. PMID: 20008566
- Mears JA, Hinshaw JE (2008) Visualization of dynamins. Methods in Cell Biol., 88, 237-256. PMID: 18617037
- Mears JA, Ray P, Hinshaw JE (2007) A corkscrew model for dynamin constriction. Structure, 15: 1190-1202. PMID: 17937909
- Ingerman E, Perkins EM, Marino M, Mears JA, McCaffery JM, Hinshaw JE, Nunnari J (2005) Dnm1 forms spirals that are structurally tailored to fit mitochondria. J. Cell Biol., 170: 1021-1027. PMID: 16186251
Papers from Jason's Graduate Studies
- Mears JA*, Sharma MR*, Gutell RR, McCook AS, Richardson PE, Caulfield TA, Agrawal RK and Harvey SC (2006) A structural model for the large subunit of the mammalian mitochondrial ribosome. J. Mol. Biol., 358: 193-212. PMID: 16510155
- Mears JA, Stagg SM, Harvey SC (2005) Modeling large RNA assemblies using a reduced representation. In Handbook of RNA Biochemistry. Eds. Hartmann, RK, Bindereif, A, Schon, A and Westof, E. Wiley-VCH, Weinheim, Germany, pp. 546-559. Link
- Ivanov VI, Mears JA (2004) Using crosslinks to study ribosomal dynamics. J. Biomol. Struct. Dyn., 21: 691-698. PMID: 14769062
- Stagg SM*, Mears JA*, Harvey SC (2003) A Structural Model for the Assembly of the 30S Subunit of the Ribosome. J. Mol. Biol., 328: 49-61. PMID: 12683996
- Mears JA, Cannone JJ, Stagg SM, Gutell RG, Agrawal RK, Harvey SC (2002) Modeling a minimal ribosome based on comparative sequence analysis. J. Mol. Biol., 321: 215-234. PMID: 12144780